Complete Sequence of Broad-Host-Range Plasmid pRIO-5 Harboring the Extended-Spectrum-β-Lactamase GeneblaBES-1

Abstract

ABSTRACTBroad-host-range plasmid pRIO-5, harboring the extended-spectrum β-lactamaseblaBES-1gene inSerratia marcescens, was fully sequenced. Analysis of the 12,957-bp sequence of this IncP6-type plasmid revealed that theblaBES-1gene was associated with two copies of the insertion sequence IS26. The promoter responsible for theblaBES-1expression was hybrid, made of a −35 box located inside the inverted repeat of IS26and a −10 box inside a remnant of an insertion sequence.