Multiplex Reverse Transcription-PCR for Simultaneous Detection of Beet Necrotic Yellow Vein Virus , Beet Soilborne Virus , and Beet Virus Q and Their Vector Polymyxa betae KESKIN on Sugar Beet-Reference-Cited by-同舟云学术

Multiplex Reverse Transcription-PCR for Simultaneous Detection of Beet Necrotic Yellow Vein Virus , Beet Soilborne Virus , and Beet Virus Q and Their Vector Polymyxa betae KESKIN on Sugar Beet

Published:2003-04 Issue:4 Volume:69 Page:2356-2360
ISSN:0099-2240
Container-title:Applied and Environmental Microbiology
language:en
Short-container-title:Appl Environ Microbiol

Author:

Meunier Alexandre¹,Schmit Jean-François¹,Stas Arnaud¹,Kutluk Nazli²,Bragard Claude¹

Affiliation:

1. Unité de Phytopathologie-UCL-AGRO-BAPA, B-1348 Louvain-la-Neuve, Belgium

2. Plant Protection Department, Agricultural Faculty, Gaziosmanpasa University, 60240 Tasliciftlik, Tokat, Turkey

Abstract

ABSTRACT Three soilborne viruses transmitted by Polymyxa betae KESKIN in sugar beet have been described: Beet necrotic yellow vein virus (BNYVV), the agent of rhizomania, Beet soilborne virus (BSBV), and Beet virus Q (BVQ). A multiplex reverse transcription-PCR technique was developed to simultaneously detect BNYVV, BSBV, and BVQ, together with their vector, P. betae . The detection threshold of the test was up to 128 times greater than that of an enzyme-linked immunosorbent assay. Systematic association of BNYVV with one or two different pomoviruses was observed. BVQ was detected in samples from Belgium, Bulgaria, France, Germany, Hungary, Italy, Sweden, and The Netherlands but not in samples from Turkey.

Publisher

American Society for Microbiology

Subject

Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology

Link

https://journals.asm.org/doi/pdf/10.1128/AEM.69.4.2356-2360.2003

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