Selenocysteine in Trypanosoma evansi: Identification of the Genes selb, selc, seld, pstk, seltryp and the Selenophosphate Synthetase Protein-Reference-Cited by-同舟云学术

Selenocysteine in Trypanosoma evansi: Identification of the Genes selb, selc, seld, pstk, seltryp and the Selenophosphate Synthetase Protein

Published:2021 Issue: Volume:60 Page:21-29
ISSN:1689-0027
Container-title:Acta Protozoologica
language:en
Short-container-title:Acta Protozool.

Author:

Tavares Kaio Cesar Simiano¹,Casagrande Dambrós Maria Gabriela²,Antunes André Saraiva Leão³,Danziato Pietro Martin³,Stoco Patricia Hermes⁴,Schlindwein Aline Daiane⁴,Moreira Renato Simões⁵,Miletti Luiz Claudio²^ORCID

Affiliation:

1. Laboratório de Hemoparasitas e Vetores Centro de Ciências Agroveterinárias (CAV), Universidade do Estado de Santa Catarina (UDESC).; Experimental Biology Center (NUBEX), Universidade de Fortaleza

2. Laboratório de Hemoparasitas e Vetores Centro de Ciências Agroveterinárias (CAV), Universidade do Estado de Santa Catarina (UDESC)

3. Experimental Biology Center (NUBEX), Universidade de Fortaleza

4. Laboratório de Protozoologia, Universidade Federal de Santa Catarina

5. Laboratório de Hemoparasitas e Vetores Centro de Ciências Agroveterinárias (CAV), Universidade do Estado de Santa Catarina (UDESC); Instituto Federal de Santa Catarina (IFSC)

Abstract

Selenoproteins have been described in all three domains of life and their function has been mainly associated with oxidative stress defense. Canonical elements required for selenoprotein production have been identified in members of the kinetoplastid group supporting the existence of a complete selenocysteine synthesis pathway in these organisms. Currently, nothing is known regarding the selenocysteine pathway in Trypanosoma evansi. In this study, we identified the expression of the elements selB, selC, selD, PSTK and selTRYP at the mRNA level in T. evansi. All translated proteins (selD, PSTK, selTRYP and selB) have the domains predicted and higher identity with Trypanosoma brucei. gambiense. The selenophosphate synthetase protein was localized in the cytoplasm. Our results support the existence of an active selenocysteine pathway in T. evansi.

Funder

Conselho Nacional de Desenvolvimento Científico e Tecnológico

Publisher

Uniwersytet Jagiellonski - Wydawnictwo Uniwersytetu Jagiellonskiego

Subject

General Agricultural and Biological Sciences

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