The possibility of a halal mix probiotic medium for the cultivation of Lactobacillus plantarum N16 and Saccharomyces cerevisiae

Author:

Marlida Yetti,Harnentis ,Azizah ,Shafan Nur Yuliaty,Adzitey FrederickORCID,Julmohammad NorlizaORCID,Huda NurulORCID

Abstract

This study aimed to determine the effects of interaction between media type (halal mix preparation) and culture mixtures of Lactobacillus plantarum N16 and Saccharomyces cerevisiae (probiotics). A completely randomised factorial design (CRFD) consisting of 2 factors and three replications was used, where factor A was a mixture of Lactobacillus plantarum N16 and Saccharomyces cerevisiae at a ratio of 1:1 (A1); 1:2 (A2) and 2:1 (A3) and factor B was the type of growth media, that is, control (B1), whey tofu, molasses, and fish waste flour (B2), and coconut water, onggok flour and shrimp waste flour (B3). The variables measured were viability, cell biomass, and pH. The results showed interactions between factors A and B, which were significantly different (p <0.05) in terms of viability, cell biomass, and pH. Based on the results of the study, it can be concluded that the mixture of Lactobacillus plantarum N16 and Saccharomyces cerevisiae at a ratio of 2:1 (A3), using coconut water, onggok flour, and shrimp waste flour (B3) as medium and incubated at 36 °C for 24 hours was the best medium. It had a 2.37 viability, 42.33 mg/ml biomass cell, and a pH of 2.37.

Publisher

HACCP Consulting

Subject

Food Science

Reference31 articles.

1. Hill, C., Guarner, F., Reid, G., Gibson, G. R., Merenstein, D. J., Pot, B., Morelli, L., Canani, R. B., Flint, H. J., Salminen, S., Calder, P. C., & Sanders, M. E. (2014). The International Scientific Association for Probiotics and Prebiotics consensus statement on the scope and appropriate use of the term probiotic. In Nature Reviews Gastroenterology & Hepatology (Vol. 11, Issue 8, pp. 506–514). Springer Science and Business Media LLC. https://doi.org/10.1038/nrgastro.2014.66

2. Marlida, Y., Huda, N., Harnentis, Shafan Nur, Y., Mekar Lestari, N., Adzitey, F., & Sulaiman, M. R. (2021). Potential probiotic yeast isolated from an Indonesian indigenous fermented fish (Ikan Budu). In Potravinarstvo Slovak Journal of Food Sciences (Vol. 15, pp. 460–466). HACCP Consulting. https://doi.org/10.5219/1544

3. Revolledo, L., Ferreira, A. J. P., & Mead, G. C. (2006). Prospects in Salmonella Control: Competitive Exclusion, Probiotics, and Enhancement of Avian Intestinal Immunity. In Journal of Applied Poultry Research (Vol. 15, Issue 2, pp. 341–351). Elsevier BV. https://doi.org/10.1093/japr/15.2.341

4. Harnentis, H., Marlida, Y., Nur, Y. S., Wizna, W., Santi, M. A., Septiani, N., Adzitey, F., & Huda, N. (2020). Novel probiotic lactic acid bacteria isolated from indigenous fermented foods from West Sumatera, Indonesia. In Veterinary World (Vol. 13, Issue 9, pp. 1922–1927). Veterinary World. https://doi.org/10.14202/vetworld.2020.1922-1927

5. Giannenas, I., Papadopoulos, E., Tsalie, E., Triantafillou, E., Henikl, S., Teichmann, K., & Tontis, D. (2012). Assessment of dietary supplementation with probiotics on performance, intestinal morphology and microflora of chickens infected with Eimeria tenella. In Veterinary Parasitology, (Vol. 188, Issue. 1-2, pp. 31–40). Elsevier Netherland. https://doi.org/10.1016/j.vetpar.2012.02.017

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