Morphological and Functional Characteristics of the Microenvironment After Pleural Implantation of Biosynthetic Materials-Reference-Cited by-同舟云学术

Morphological and Functional Characteristics of the Microenvironment After Pleural Implantation of Biosynthetic Materials

Published:2023-03-04 Issue:1 Volume:101 Page:48-54
ISSN:2542-1506
Container-title:Tuberculosis and Lung Diseases
language:
Short-container-title:Tuberk. bolezni lëgk.

Author:

Demyashkin G. A.¹^ORCID,Tokaev T. K.²^ORCID,Bikbaev A. S.²,Sinitsyn M. V.³^ORCID,Grigoryev T. E.⁴^ORCID,Zagoskin Yu. D.⁴^ORCID

Affiliation:

1. I.M. Sechenov First Moscow State Medical University

2. National Medical Research Center of Phthisiopulmonology and Infectious Diseases, Russian Ministry of Health

3. National Medical Research Center of Phthisiopulmonology and Infectious Diseases, Russian Ministry of Health; Pirogov Russian National Research Medical University

4. Kurchatov Institute National Research Center

Abstract

The objective: to study morphological changes in tissues after implantation of a bioresorbable material synthesized on the basis of polylactide (PLA) and polycaprolactone (PCL) in various ratios.Subjects and Methods. Laboratory animals Chinchilla lanigera (n = 12) underwent intrapleural implantation with 2% PLA/PCL 1/1 and 4% PLA/PCL 1/3 materials. Morphometric assessment was carried out in14 and 28 weeks, the animals were withdrawn from the experiment by an anesthetic overdosing.Results. When studying tissues adjacent to the implant regardless of differences in its composition, similar morphological signs were observed. A fibrous capsule formed around the implants, and it was more pronounced in 4% of PLA/PCL 1/3. In the structure of the implants, we noted the growth of loose and dense unformed fibrous connective tissue with blood vessels with a significant replacement of the bioresorbable polymer material (by 2/3). Lymphocytes, plasma cells, single macrophages, and multinucleated cells were predominantly located at the implant/tissue interface. In all studied samples, no necrosis was observed in the implantation bed and surrounding tissue.Conclusion. The data obtained confirm the biosafety of biomaterials and demonstrate the potential for their use in surgical treatment of humans.

Publisher

LLC "Medical Knowledge and Technologies"

Subject

General Medicine

Reference12 articles.

1. Krasnikova E.V., Popova L.A., Aliev V.K., Tarasov R.V., Turovtseva Yu.V., Ibriev A.S., Sadovnikova S.S., Bagirov M.A. A сase of bilateral silicone plombage and valve bronchoblocation in a patient with widespread fibrocavernous drug resistant tuberculosis of the operated lung. Novosti Khirurgii. 2019, vol. 27, no. (4), pp. 461-468. (In Russ.) doi:10.18484/2305-0047. 2019.4.461.

2. Lovacheva O.V., Bagirov M.A., Bagdasaryan T.R., Krasnikova E.V., Shergina E.A., Gritsay I.Yu. Use of endobronchial valves and extrapleural plombage for treatment of bilateral massive cavities in a female patient with multiple drug resistant pulmonary tuberculosis. Tuberculosis and Lung Diseases, 2017, vol. 95, no. 9, pp. 60-67. (In Russ.) doi: 10.21292/2075-1230-2017-95-960-67.

3. Sinitsyn M.V., Agkatsev T.V., Reshetnikov M.N., Pozdnyakova E.I., Itskov A.V., Gazdanov T.A., Plotkin D.V. Extrapleural pneumolysis with plombage for cavitary pulmonary tuberculosis treatment. Khirurg, 2018, no. 1-2, pp. 54-63. (In Russ.)

4. Sinitsyn M.V., Kalinina M.V., Belilovsky E.M., Galstyan A.S., Reshetnikov M.N., Plotkin D.V. The treatment of tuberculosis under current conditions. Terapevticheskiy Arkhiv, 2020, vol. 92, no. 8, pp. 86-94. (In Russ.) doi: 10.26442/00403660.2020.08.000762.

5. Barbeck M., Serra T., Booms P., Stojanovic S., Najman S., Engel E., Sader R., Kirkpatrick C. J., Navarro M., Ghanaati S. Analysis of the in vitro degradation and the in vivo tissue response to bi-layered 3D-printed scaffolds combining PLA and biphasic PLA/bioglass components - Guidance of the inflammatory response as basis for osteochondral regeneration // Bioact. Mater. – 2017. Vol. 2 № 4. – Р. 208-223. doi: 10.1016/j.bioactmat.2017.06.001. PMID: 29744431; PMCID: PMC5935508.