Here we present a rapid (<4 hours from sample in to result out) culture-independent Bcc NAD method, incorporating a quantitative real-time Polymerase Chain Reaction (qPCR) assay. This method can be used to detect and simultaneously identify the Bcc species directly from water samples. This culture-independent Bcc NAD method is validated to the testing method equivalent of the ISO/TS 12869:2019 standard (a widely used rapid culture-independent NAD method for detecting Gram-negative Legionella species in water) in terms of specificity, sensitivity, accuracy and turnaround time to result compared to Bcc microbial culture diagnostic methodologies. We propose that the rapid culture-independent Bcc NAD method presented could gain widespread acceptance and be applied as a valuable diagnostic tool supporting a water risk management strategy in the pharmaceutical sector to assure the safety and quality of these water systems and water used for manufacturing. The development and validation of this culture-independent Bcc NAD method has been published in: