This protocol describes a method to analyze the effect of PCB52 inhalation on brain cells in adolescent rats. PCB52 (2,2’,5,5’-tetrachlorobiphenyl) was administered to adolescent rats via a nose-only apparatus with PCB52-laden air. The exposure was 4 hours a day for 28 consecutive days. Brains were collected, the cerebellum region was frozen, and tissue sections were cut and stained to label apoptotic cells using PSvue, microglia using anti-IBA1, and astrocytes using anti-GFAP. Using a Leica TCS SP8 confocal microscope, Z-Stack images were taken and analyzed for differences in cell density and apoptosis within the distinct cerebellar layers.