Analysis of DNA methylation profiles confirms a high proportion of immune cells in glioblastoma samples-Reference-Cited by-同舟云学术

Analysis of DNA methylation profiles confirms a high proportion of immune cells in glioblastoma samples

Published:2023-11-29 Issue:3 Volume:10 Page:15-21
ISSN:2413-5496
Container-title:Russian Journal of Pediatric Hematology and Oncology
language:
Short-container-title:Ross. ž. det. gematol. onkol.

Author:

Petrova E. I.¹^ORCID,Olkhova L. V.²^ORCID,Galstyan S. A.¹^ORCID,Telysheva E. N.¹^ORCID,Zheludkova O. G.³^ORCID,Ryzhova M. V.¹^ORCID

Affiliation:

1. N.N. Burdenko National Medical Research Center of Neurosurgery, Ministry of Health of Russia

2. Russian Children’s Clinical Hospital – Branch of the N.I. Pirogov Russian National Research Medical University, Ministry of Health of Russia

3. Scientific and Practical Center for Specialized Medical Care for Children named after V.F. Voyno-Yasenetsky Moscow Healthcare Department; Diagnostic and Treatment Center of International Institution for Biological Systems named after Sergey Berezin

Abstract

Glioblastomas are malignant tumors that belong to the central nervous system and are challenging to diagnose due to their significant intratumoral heterogeneity, which makes molecular testing and diagnosis confirmation particularly difficult. In addition to identifying typical genetic mutations such as IDH, H3F3A G34 and K27, WHO recommendations emphasize the importance of analyzing the tumor epigenome to define its class based on DNA methylation patterns and methylation status of specific genomic regions, particularly the MGMT promoter region. Based on our clinical experience, molecular genetic studies sometimes yield contradictory results due to the heterogeneous cellular composition of glioblastomas. In this study, we present a series of observations made on 35 glioblastoma samples in which we compare the morphological features and the results of cell type detection by deconvolution method based on total DNA methylation profiles. Our results suggest that samples of mesenchymal class glioblastomas may contain over 50 % non-tumor immune cells, which should be considered in genetic testing of these tumors.

Publisher

OOO Grafika

Subject

Oncology,Hematology,Pediatrics, Perinatology and Child Health

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