Genotyping of red tilapia strains in Central Luzon Philippines targeting ESR1 gene

Abstract

Tilapia is an economically important commodity worldwide. Marker-assisted selection (MAS) has been practiced in livestock culture to ensure the selection of a specific trait that will benefit the culture system. This study assessed the allelic variation in six red tilapia strains in Central Luzon, Philippines. Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (PCR-RFLP) was done to evaluate the genotypes of six strains of red tilapia using intron one portion of the chromosome one of the estrogen receptor 1 (ESR1) gene. The target gene was amplified using primers designed in previous study and then subjected to RFLP using PvuII restriction enzyme. Single Nucleotide Polymorphisms (SNPs) were also evaluated in six DNA sequences. Results showed that 18 red tilapia samples were found to be of the AA genotype. Aligned sequences of the 122 bp ESR1 gene revealed that among six red tilapia strains, there are 117 identical pairs, three transversional pairs, and three transitional pairs. The SNPs observed can be used to identify restriction enzymes that discriminate different genotypes in the target gene. Association studies can be done to determine the genotypes that are linked to specific traits in cultured animals.