Author:
Miyake A.,Tasaka K.,Sakumoto T.,Kawamura Y.,Nagahara Y.,Aono T.
Abstract
Abstract. The releasing effects of clomiphene citrate (clomiphene) on luteinizing hormone (LH) and LH-releasing hormone (LRH) were examined in a sequential double chamber superfusion system by superfusing the mediobasal hypothalami (MBH) and/or pituitaries excised from normal female rats in dioestrus. When the MBH and the pituitary were superfused in sequence with medium containing 2 × 10−10 m oestradiol (E2), two significant peaks in LH release (60–130% increase, P < 0.05) were observed 40 min and 90 min after the administration of 3 × 10−8 mol clomiphene. Administration of clomiphene in medium without E2 induced a low peak (25–50% increase, P < 0.05) of LH released from the pituitary perfused in series with the MBH. Administration of clomiphene did not cause a marked increase of LH from the pituitary superfused alone, when superfused with or without E2 containing medium. The concentration of LRH in the efflux was significantly increased (50–100%) 40 min and 90 min after clomiphene administration when MBH was superfused with medium containing E2, whereas clomiphene had no effect when superfused with medium alone. These data indicate: 1) that clomiphene induces LRH release from the MBH, that it may induce LH release, in part, by acting directly at the pituitary level; 2) that changes in LH after clomiphene administration coincide with LRH release, and 3) that a certain concentration of E2 may be necessary for the secretion of LRH by clomiphene.
Subject
Endocrinology,General Medicine,Endocrinology, Diabetes and Metabolism
Cited by
35 articles.
订阅此论文施引文献
订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献
1. Female Infertility;Yen and Jaffe's Reproductive Endocrinology;2019
2. Female Infertility;Yen & Jaffe's Reproductive Endocrinology;2014
3. Pulsatile Luteinizing Hormone Secretion in Women with Polycystic Ovary Syndrome under Clomiphene Citrate. Differences between Responders and Nonrespondersa
,
b);Experimental and Clinical Endocrinology & Diabetes;2009-07-16
4. Place des inductions de l’ovulation;Journal de Gynécologie Obstétrique et Biologie de la Reproduction;2009-01
5. Female Infertility;Yen & Jaffe's Reproductive Endocrinology;2009