Differential reprogramming of somatic cell nuclei after transfer into mouse cleavage stage blastomeres

Author:

Eckardt Sigrid,Leu N Adrian,Kurosaka Satoshi,McLaughlin K John

Abstract

Mammalian somatic cell cloning requires factors specific to the oocyte for reprogramming to succeed. This does not exclude that reprogramming continues during the zygote and cleavage stages. The capacity or role of zygotic and cleavage stages to reprogram somatic cell nuclei is difficult to assess due to the limited development of somatic cell nuclei transplanted into cytoplasts of these stages. Alternatively, tetraploid embryos have been used to study reprogramming and can be assessed for their contribution to extra-embryonic lineages. When mouse cumulus cell nuclei transgenic for Oct4-green fluorescent protein (GFP) were injected into intact two- and four-cell stage blastomeres, manipulated embryos developed into blastocysts with expression of Oct4-GFP as observed in embryos produced by nuclear transfer into metaphase II oocytes. However, only the latter contributed to extra-embryonic tissues in day 10.5 conceptuses, with the exclusion of the somatic genome in cells originating from transfer into blastomeres already at 5.5 days post conception. Somatic nuclei transferred into cleavage stage blastomeres reinitiated expression of an embyronic-specific transgene, but lacked the extent of reprogramming required for contribution to postimplantation development, even when complemented by an embryonic genome.

Publisher

Bioscientifica

Subject

Cell Biology,Obstetrics and Gynecology,Endocrinology,Embryology,Reproductive Medicine

Cited by 14 articles. 订阅此论文施引文献 订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献

1. The Oocyte Determinants of Early Reprogramming;Epigenetic Mechanisms in Cellular Reprogramming;2014-11-18

2. Nuclear reprogramming by interphase cytoplasm of two-cell mouse embryos;Nature;2014-03-26

3. How Cell Division Facilitates Nuclear Reprogramming;Principles of Cloning;2014

4. Cloning of Medaka Fish;Principles of Cloning;2014

5. Mechanisms and Dynamics of Heterochromatin Formation During Mammalian Development;Current Topics in Developmental Biology;2013

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