Dopamine synthesis and dopamine receptor expression are disturbed in recurrent miscarriages

Author:

Gratz Michael J1,Stavrou Stavroula1,Kuhn Christina1,Hofmann Simone1,Hermelink Kerstin1,Heidegger Helene1,Hutter Stefan1,Mayr Doris2,Mahner Sven1,Jeschke Udo1,Vattai Aurelia1

Affiliation:

1. 1Department of Obstetrics and Gynecology, University Hospital, LMU Munich, Munich, Germany

2. 2Department of Pathology, University Hospital, LMU Munich, Munich, Germany

Abstract

Objectives l-dopa decarboxylase (DDC) is responsible for the synthesis of dopamine. Dopamine, which binds to the D2-dopamine receptor (D2R), plays an important role in the maintenance of pregnancy. Aim of our study was the analysis of DDC and D2R expression in placentas of spontaneous miscarriages (SMs) and recurrent miscarriages (RMs) in comparison to healthy controls. Methods Patients with SM (n = 15) and RM (n = 15) were compared with patients from healthy pregnancies (n = 15) (pregnancy weeks 7–13 each). Placental tissue has been collected from SMs and RMs from the first trimester (Department of Gynaecology and Obstetrics, LMU Munich) and from abruptions (private practice, Munich). Placental cell lines, BeWo- and JEG-3 cells, were stimulated with the trace amines T0AM and T1AM in vitro. Results Levels of DDC and D2R in trophoblasts and the decidua were lower in RMs in comparison to healthy controls. Stimulation of BeWo cells with T1AM significantly reduced DDC mRNA and protein levels. Via double-immunofluorescence, a DDC-positive cell type beneath decidual stromal cells and foetal EVT in the decidua could be detected. Conclusions Downregulation of DDC and D2R in trophoblasts of RMs reflects a reduced signal cascade of catecholamines on the foetal side.

Publisher

Bioscientifica

Subject

Endocrinology,Endocrinology, Diabetes and Metabolism,Internal Medicine

Reference28 articles.

1. The neurochemistry of Parkinson’s disease: effect of L-dopa therapy.;Journal of Pharmacology and Experimental Therapeutics,1975

2. Cloning and sequencing of human neuronal aromatic L-amino acid decarboxylase gene and its therapeutic effects on Parkinson’s disease.;Chinese Journal of Clinical Rehabilitation,2004

3. The role of thyroid hormone in trophoblast function, early pregnancy maintenance, and fetal neurodevelopment.;Journal of Obstetrics and Gynaecology Canada,2004

4. Aromatic L-amino acid decarboxylase: a neglected and misunderstood enzyme.;Neurochemical Research,1996

5. The relationship between maternal serum thyroid-stimulating immunoglobulin and fetal and neonatal thyrotoxicosis.;Obstetrics and Gynecology,2002

同舟云学术

1.学者识别学者识别

2.学术分析学术分析

3.人才评估人才评估

"同舟云学术"是以全球学者为主线,采集、加工和组织学术论文而形成的新型学术文献查询和分析系统,可以对全球学者进行文献检索和人才价值评估。用户可以通过关注某些学科领域的顶尖人物而持续追踪该领域的学科进展和研究前沿。经过近期的数据扩容,当前同舟云学术共收录了国内外主流学术期刊6万余种,收集的期刊论文及会议论文总量共计约1.5亿篇,并以每天添加12000余篇中外论文的速度递增。我们也可以为用户提供个性化、定制化的学者数据。欢迎来电咨询!咨询电话:010-8811{复制后删除}0370

www.globalauthorid.com

TOP

Copyright © 2019-2024 北京同舟云网络信息技术有限公司
京公网安备11010802033243号  京ICP备18003416号-3