Author:
Potikanond Saranyapin,Rattanachote Pinyada,Pintana Hiranya,Suntornsaratoon Panan,Charoenphandhu Narattaphol,Chattipakorn Nipon,Chattipakorn Siriporn
Abstract
The present study aimed to test the hypothesis that testosterone deprivation impairs osteoblastic insulin signaling, decreases osteoblast survival, reduces bone density, and that obesity aggravates those deleterious effects in testosterone-deprived rats. Twenty four male Wistar rats underwent either a bilateral orchiectomy (O,n=12) or a sham operation (S,n=12). Then the rats in each group were further divided into two subgroups fed with either a normal diet (ND) or a high-fat diet (HF) for 12 weeks. At the end of the protocol, blood samples were collected to determine metabolic parameters and osteocalcin ratios. The tibiae were collected to determine bone mass using microcomputed tomography and for osteoblast isolation. The results showed that rats fed with HF (sham-operated HF-fed rats (HFS) and ORX HF-fed rats (HFO)) developed peripheral insulin resistance and had decreased trabecular bone density. In ND-fed rats, only the ORX ND-fed rats (NDO) group had decreased trabecular bone density. In addition, osteoblastic insulin resistance, as indicated by a decrease in tyrosine phosphorylation of the insulin receptor and Akt, were observed in all groups except the sham-operated ND-fed rats (NDS) rats. Those groups, again with the exception of the NDS rats, also had decreased osteoblastic survival. No differences in the levels of osteoblastic insulin resistance and osteoblastic survival were found among the NDO, HFS, and HFO groups. These findings suggest that either testosterone deprivation or obesity alone can impair osteoblastic insulin signaling and decrease osteoblastic survival leading to the development of osteoporosis. However, obesity does not aggravate those deleterious effects in the bone of testosterone-deprived rats.
Subject
Endocrinology,Endocrinology, Diabetes and Metabolism
Reference54 articles.
1. Albala C Yáñez M Devoto E Sostin C Zeballos L Santos JL 1996 Obesity as a protective factor for postmenopausal osteoporosis. International Journal of Obesity and Related Metabolic Disorders 20 1027–1032.
2. Testosterone Concentrations in Women and Men With NIDDM
3. Basal plasma insulin and homeostasis model assessment (HOMA) are indicators of insulin sensitivity in cats
4. Ayaz M Akand M Kucukbagriacik Y Dursunoglu D 2015 The effects of estradiol on cardiac muscle electrophysiology in orchiectomized rat model: a new insight to side effects caused by castration. European Review for Medical and Pharmacological Sciences 19 2866–2874.
5. Lower Endogenous Androgen Levels and Dyslipidemia in Men with Non-Insulin-Dependent Diabetes Mellitus
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