STEROID BIOSYNTHESIS BY ADRENAL AND OVARIAN TISSUE IN CONGENITAL ADRENAL HYPERPLASIA

Abstract

ABSTRACT Fresh adrenal tissue from a 5 year old boy with congenital adrenal hyperplasia and ovarian tissue from an untreated adult female pseudohermaphrodite were incubated with radioactive steroids. Adrenal tissue incubated with 4-14C-progesterone produced 17-hydroxyprogesterone, 21-deoxycortisol and testosterone in high yield. The ovarian tissue gave evidence of 17-hydroxylating, 20-reducing, 3β-ol dehydrogenase, 17β-hydroxysteroid dehydrogenase, and desmolase activities.