Author:
Jarlstedt Jan,Nilsson Lars,Hamberger Lars,Ahrén Kurt
Abstract
ABSTRACT
In vivo and in vitro effects of FSH and LH on in vitro incorporation of [3H]uridine into RNA of the prepubertal rat ovary have been studied. RNA was fractionated with composite agarose-polyacrylamide gel electrophoresis. When FSH was injected into the prepubertal rats 4 h before incubation of the ovaries, the incorporation of labelled uridine into total RNA was decreased showing relatively more radioactivity concentrated to the RNA fractions lighter than 28S as compared to the controls. These effects were not seen when FSH was added to the incubation medium in vitro. When LH was added in vitro to the isolated ovaries a higher percentage of incorporated radioactivity was concentrated in the RNA fractions heavier than 28S without any change in the incorporation of [3H]uridine into total RNA. LH administered in vivo 30 min before incubation of the ovaries gave the same change in the labelling pattern between the RNA fractions as LH in vitro but in addition showed a decreased incorporation of radioactivity into total RNA.
The in vitro effects of cyclic 3′,5′-AMP were also studied. When prepubertal rat ovaries were incubated in 10 mmol/l of this cyclic nucleotide, the incorporation of [3H] uridine into total RNA was decreased without any change in the labelling pattern.
Subject
Endocrinology,General Medicine,Endocrinology, Diabetes and Metabolism
Cited by
9 articles.
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