Author:
Goldzieher Joseph W.,Nakamura Yutaka
Abstract
ABSTRACT
A method suitable for the routine determination of urinary pregnanediol and pregnanetriol has been devised. It involves glucuronidase hydrolysis, extraction, and chromatography on disposable 1-g silica gel columns. The specificity of the pregnanediol determination is increased by acetylation and rechromatography on the same type of column. Sensitivity of the method is doubled by the use of sulfuric acid saturated with SO2 gas as the chromogenic agent.
Subject
Endocrinology,General Medicine,Endocrinology, Diabetes and Metabolism
Cited by
45 articles.
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