IN VITRO STOFFWECHSEL VON [4-14C]-TESTOSTERON IN VESICULARDRÜSEN VON RATTEN

Abstract

ABSTRACT Experiments to elucidate the in vitro metabolism of [4-14C] -testosterone in seminal vesicles of rats are described. Besides unchanged testosterone, the following metabolites were isolated and identified by their chromatographic behaviour: 3α,17β-dihydroxy-5α-androstan, androst-4-ene-3,17-dione, 17β-hydroxy-5α-androstan-3-one, 3β-hydroxy-5α-androstan-17-one, 5α-androstane-3,17-dione. The first 3 of these compounds were also identified by the inverse isotope dilution technique. The reduction in ring A yields 5α-metabolites only.