Radioimmunoassay of three oestrogens and three androgens in the same plasma sample after extraction and chromatographic separation

Abstract

Abstract. The plasma levels of oestrone (Oe1), 17β-oestradiol (Oe2), oestriol (Oe3), testosterone (T), 5α-dihydrotestosterone (DHT) and androstenedione (A) were assayed by RIA in plasma obtained from peripheral venous blood. The hormones are isolated from the plasma extract, first by Sephadex LH-20 column chromatography (Oe2/Oe3/Oe1, T, DHT, A) and after Oe1, T, DHT, A by TLC on silica gel 60 F254. The accuracy, reproducibility and sensitivity of the method make it satisfactory for clinical studies.