DOUBLE ISOTOPE DILUTION DERIVATIVE TECHNIQUE FOR TESTOSTERONE GLUCURONOSIDE IN URINE

Abstract

ABSTRACT A double isotope dilution derivative technique for the determination of testosterone glucuronoside in urine has been developed. This is a highly specific and sensitive method which is applicable to the measurement of testosterone in submicrogram amounts in urine and other biological fluids. This method has been used to study normal prepubertal and pubertal males, normal females and subjects with gonadal dysgenesis, Cushing's syndrome, congenital adrenal hyperplasia, idiopathic hirsutism, Addison's disease and hypogonadism. The patients were studied after stimulation and suppression of gonads or adrenal glands to delineate the source of the androgen and its physiological control mechanism. The testosterone production of normal males determined by the method presented herein corresponds to results of others.