Author:
Sulimovici S.,Lunenfeld B.,Shelesnyak M. C.
Abstract
ABSTRACT
A method is described for the assay of urinary pregnanediol and allopregnanediol which employs acid hydrolysis of pregnanediol glucuronide, ascending thin-layer chromatography and spectrophotometric quantitation of the eluted pregnanediol and allopregnanediol. The reliability of the method was established by analysis of infra-red and UV absorption spectra, melting point determination, recovery experiments, duplicate assays and comparison with the Klopper method. The method has advantages in that it is rapid and requires small urine samples.
Subject
Endocrinology,General Medicine,Endocrinology, Diabetes and Metabolism
Cited by
17 articles.
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