A hormonally controlled serum factor stimulating the thymidine uptake into lectin-activated lymphocytes

Author:

Thieriot-Prévost Geneviève,Schimpff Rose-Marie,Sharif A.

Abstract

Abstract. The in vitro stimulation of [3H]thymidine uptake into lectin-activated lymphocytes in the presence of various sera was studied. The mean precision of the assay is 5%, and the study of the confidence intervals shows variations from 4% to 12%. Compared to a normal reference serum (fixed as 1 U/ml), the serum thymidine uptake stimulating activity (mean ± sem) was 1.04 ± 0.07 U/ml in normal adult males, 2.63 ± 0.48 U/ml in acromegalic patients, 1.51 ± 0.13 U/ml in constitutional dwarfism and 0.37 ± 0.04 U/ml in untreated hypopituitary dwarfism with a significant difference between the groups (P < 0.001). In patients with hypopituitarism a single im hGH dose (6 mg/m2) increased the thymidine uptake stimulating activity of serum within 24 to 48 h following injection. The effects of directly adding hGH, insulin and T3 to the assay, have been studied: pharmacological concentrations are required to produce only a slight effect. Physiological concentration of a purified preparation of somatomedin A stimulated thymidine uptake and its effect is increased in the presence of serum. These data demonstrate that [3H]thymidine uptake into lectin-activated lymphocytes is stimulated by a GH-dependent serum factor. The data suggest that this method should be proposed for an accurate and sensitive biological evaluation of serum thymidine uptake stimulating activity.

Publisher

Bioscientifica

Subject

Endocrinology,General Medicine,Endocrinology, Diabetes and Metabolism

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