PAPIERELEKTROPHORETISCHE AUFSPALTUNG GONADOTROPER SUBSTANZEN

Abstract

ABSTRACT The paperelectrophoresis of different charges of one chorion-gonadotrophin preparation and two charges of a serum-gonadotrophin preparation at different pH-levels showed in the course of systematic tests of electrophoresis-strips in the bio-test (weight-test of the ovary, uterus and the ventral lobe of the prostate-gland of intact infantile rats and the ovary weight-test of hypophysectomized infantile rats) with special consideration for the histology of the ovaries an exactly localised, biologically active complex. A suitable dose has been used for the different test-organs which resulted from the bioassays of the different charges. A weight-curve with multiple peaks at systematical tests is not caused by many or biologically different fractions but the result of a relative over-dosage. Through histological slides of the ovaries of hypophysectomized infantile rats a slight FSH-activity could be demonstrated in the center of the biological maximum for the chorion-gonadotrophin with higher dosage. The migratory speed of the biological activity at the same pH-level was almost the same for the different charges of the same chorion-gonadotrophin preparation, of which the mucopolysaccharide-spectrums in the electropherogram after staining with Ninhydrin, Amido-Black 10 B and Schiff's Stain differ partly to a higher degree. The biological activity therefore, migrated in differently constituted mucopolysaccharide-complexes.