Author:
Kushinsky Stanley,Tang Jane (Wu)
Abstract
ABSTRACT
A method is described for the extraction and separation of free and conjugated oestrogens in urine. Celite 545, a diatomaceous earth, is coated with a high molecular weight secondary amine (Amberlite LA-1, Rohm and Haas, Phila., Pa.) and is used as a chromatographic support for the separation of various free and conjugated oestrogens in urine and in extracts of urine. Elution is carried out with extremely dilute acids. Optimum separation was obtained with a column of Celite coated with LA-1 (formate) in which the conjugated oestrogens in a concentrated extract of urine were eluted with a linear gradient (in the mixing chamber) of formic acid (pH 3.10 to 5.04).
Subject
Endocrinology,General Medicine,Endocrinology, Diabetes and Metabolism
Cited by
6 articles.
订阅此论文施引文献
订阅此论文施引文献，注册后可以免费订阅5篇论文的施引文献，订阅后可以查看论文全部施引文献