Author:
Kohl K.-H.,Vecsei P.,Abdelhamid S.
Abstract
ABSTRACT
Specific antisera against tetrahydroaldosterone (TH-Aldo) were raised in two white New Zealand rabbits. 3α,5β-TH-aldo-20-oxime-bovine-serum albumin complex was used as antigen. The resulting titers were 1:18 000 and 1:16 000. Except tetrahydrocortisol (THF) (0.23%) and tetrahydro-18-hydroxy-11-dehydrocorticosterone (18-OH-THA) (3.2 %), all steroids and steroid metabolites gave negligible cross-reactions. Immunograms of the paper chromatograms made from the n-butanol-extract of the urines, as well as after β-glucuronidase treatment and dichlormethane extraction, were studied to further define the specificity of the antiserum. Antibody H1 (used in this study) reacted with aldosterone-18-gluc., a TH-aldosterone-glucuronide (probably the 21-glucuronide) and an unidentified less polar material.
Two methods were developed:
a) TH-Aldo-glucuronidc(s) estimation after ethylacetate pre-extraction as a rapid screening test of endogenous aldosterone production.
b) estimation of TH-aldosterone using one chromatographic system.
The results of method a) showed a significant correlation with the values obtained by technique b). Normal values (method b) were 25.88 ± 16.50 μg/24h (range 9.5–64.8 μg/24h).
A significant correlation was also shown between the TH-aldo (technique b) and 18-gluc. values.
Subject
Endocrinology,General Medicine,Endocrinology, Diabetes and Metabolism
Cited by
38 articles.
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