PURIFICATION OF GONADOTROPHIN FROM HUMAN MENOPAUSAL URINE

Abstract

ABSTRACT Gonadotrophins were prepared from human postmenopausal urine with the kaolin-acetone method and purified by adsorption of impurities on diethylamine ethyl cellulose (DEAE-C). The last step, namely chromatography on the column of permutit, resulted in a significantly improved specific activity. The immunoelectrophoresis showed that the purified HMG is not homogeneous and that the constituents are glycoproteins which migrate into the region α1, α2 and β globulins.