SUBCELLULAR DISTRIBUTION OF RADIOACTIVITY IN THE PROSTATE GLAND FOLLOWING THE SINGLE INJECTION OF [1,2-3H]TESTOSTERONE

Abstract

ABSTRACT Single injections of [1,2-3H] testosterone (100 μCi/kg) to normal mice revealed substantial amounts of radioactivity in the anterior lobes of the prostate gland. Levels of radioactivity were considerably higher in organs obtained from mice injected by the intraperitoneal route than levels in organs obtained from mice that had received subcutaneous injections. At five minutes following the injection of tritiated testosterone most of the radioactivity was found in the 1000 × g pellet. Lesser amounts were found in the 100 000 × g supernatant. Castration caused an increase in the amount of radioactivity found in the prostate gland. However this increased uptake was not observed until at least three days following orchidectomy.