Characterization of morphology and angiogenesis in follicles of mares during spring transition and the breeding season

Abstract

The mare is a seasonal breeder and undergoes a period of ovarian transition in spring between winter anoestrus and cyclicity. During spring transition LH concentrations are low and many mares have successive large anovulatory follicular waves which reach the size of preovulatory follicles. Follicular angiogenesis is essential for growth and health of preovulatory follicles. The aim of the present study was to investigate the morphology and vascularity of transitional anovulatory follicles. On gross inspection, the wall of transitional follicles was visibly less well vascularized than that of preovulatory follicles. Histologically, it could be seen that the theca was only poorly developed in transitional follicles. Immunostaining for factor VIII showed that there were significantly (P < 0.05) fewer blood vessels in the theca of transitional follicles. There was substantially less (P < 0.001) proliferative activity, measured by immunostaining for Ki67, in the endothelial cells and granulosa cells of transitional follicles compared with preovulatory follicles. Preovulatory follicles had a heavy band of immunostaining in the theca for vascular endothelial growth factor (VEGF), whereas staining was sparse in the transitional follicles. It was concluded that the poor vascularity and development of the theca layer in transitional follicles could be related to low circulating LH, and possibly other trophic hormones, and are likely to be the key factors in explaining the steroidogenic incompetence of transitional anovulatory follicles.