Secretory and cytosolic phospholipase A2 activities and expression are regulated by oxytocin and estradiol during labor

Abstract

The release of arachidonic acid from membrane glycerophospholipids through the action of phospholipases (PLs) is the first step in the biosynthesis of prostaglandins (PGs). In reproductive tissues, the most important PLs are cytosolic PLA2(cPLA2) and types IIA and V of the secretory isoform (sPLA2). The aim of this work was to investigate the role of ovarian steroid hormones and oxytocin (OT) in the regulation of rat uterine PLA2activity and expression during pregnancy and labor. The activity of sPLA2increased near labor, whereas cPLA2activity augmented towards the end of gestation. The levels of sPLA2IIA and cPLA2mRNA showed an increase before labor (P<0.05, day 21), whereas sPLA2V mRNA was not regulated during pregnancy. The administration of atosiban (synthetic OT antagonist) together with tamoxifen (antagonist of estrogen receptors) was able to decrease cytosolic and secretory PLA2activities, diminish the expression of sPLA2IIA and cPLA2, as well as decrease PGF2αproduction before the onset of labor (P<0.01). The ovarian steroid did not affect PLA2during pregnancy. Collectively, these findings indicate that in the rat uterus, both 17β-estradiol and OT could be regulating the activity and the expression of the secretory and the cytosolic isoforms of PLA2, thus controlling PGF2αsynthesis prior to the onset of labor.