Quercetin improves white adipose tissue redox homeostasis in ovariectomized rats

Author:

Matta Leonardo123ORCID,Breves Cinthia1,Fonte Boa Luiz1,Domingos Aina Eiras1,Faria Caroline C14,Souza Itanna1,Correia Lima-Junior Niedson1,Rocha Anna Paola Trindade5,Gregório Bianca M6,Carvalho Denise Pires1,Ferreira Andrea C F15,Nascimento José Hamilton Matheus1,Maciel Leonardo7,Fortunato Rodrigo S1ORCID

Affiliation:

1. Carlos Chagas Filho Institute of Biophysics, Federal University of Rio de Janeiro, Rio de Janeiro, Brazil

2. Institute for Cardiovascular Prevention, Ludwig-Maximilians-University Munich, Munich, Germany

3. Institute for Diabetes and Cancer, Helmholtz Center Munich, Munich, Germany

4. UMR9019 CNRS, Université Paris-Saclay, Institut Gustave Roussy, Villejuif, France

5. Josué de Castro Institute of Nutrition, Federal University of Rio de Janeiro, Rio de Janeiro, Brazil

6. Urogenital Research Unit, State University of Rio de Janeiro, Rio de Janeiro, Brazil

7. NUMPEX, Duque de Caxias Campus, Federal University of Rio de Janeiro, Rio de Janeiro, Brazil

Abstract

Estrogen deficiency is a well-known hallmark of menopause and is associated with oxidative stress and metabolic dysfunction. Quercetin (Q), a flavonoid found in fruits and vegetables, has demonstrated anti-inflammatory effects in experimental models of metabolic disorders. In this study, we aimed to investigate the effects of quercetin on retroperitoneal white adipose tissue (rWAT) redox homeostasis and systemic metabolic parameters in ovariectomized (OVX) rats. Female Wistar rats at 3 months old were divided into the following experimental groups: sham-operated treated with vehicle (DMSO 10% + PBS – 1 mL/kg); OVX (vehicle treated) and OVX-Q (25 mg/kg) – via oral gavage, daily for 5 weeks. Q did not prevent weight gain but improved glucose tolerance and blood cholesterol profile, and attenuated uterine atrophy in OVX rats. Furthermore, Q had a protective effect on rWAT, once the OVX-Q group presented lower oxidative stress levels, and reduced levels of the pro-inflammatory cytokine tumor necrosis factor alpha, compared to the OVX group. Q improved antioxidant enzyme activities such as superoxide dismutase and catalase and decreased reactive oxygen species production, in OVX-Q rats. It was followed by increased levels of total thiol content and lower lipid peroxidation. Moreover, Q reduced senescent-related genes p16INK4a and p19ARF expression which were higher in the OVX group. In conclusion, quercetin supplementation improved redox homeostasis and reduced senescence-related markers, and inflammation in rWAT, which was reflected in preserved systemic metabolic health parameters in OVX rats. These findings suggest that quercetin may have therapeutic potential for the management of metabolic disorders associated with menopause-induced estrogen deficiency.

Publisher

Bioscientifica

Subject

Endocrinology,Endocrinology, Diabetes and Metabolism

Reference64 articles.

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