Author:
Gong Jishang,Zhang Quanwei,Wang Qi,Ma Youji,Du Jiaxiang,Zhang Yong,Zhao Xingxu
Abstract
™©¾‡↕♂♀∠∇±°éÑ
PIWI-interacting RNAs (piRNA) are small non-coding RNA molecules expressed in animal germ cells that interact with PIWI family proteins to form RNA- protein complexes involved in epigenetic and post- transcriptional gene silencing of retrotransposons and other genetic elements in germ line cells, including reproductive stem cell self- sustainment, differentiation, meiosis and spermatogenesis. In the present study, weperformed high- throughput sequencing of piRNAs in testis samples from yaks in different stages of sexual maturity. Deep sequencing of the small RNAs (18 to 40 nt in length)yielded 4,900,538 unique reads from a total of 53,035,635 reads. We identified yak small RNAs (18- 30 nt) and performed functional characterization. Yak small RNAs showed a bimodal length distribution, with two peaks at 22 nt and >28 nt. More than 80% of the 3,106,033 putative piRNAs were mapped to 4,637 piRNA- producing genomic clusters using RPKM. 6,388 candidate piRNAs were identified from clean reads and the annotations were compared with the yak reference genome repeat region. Integrated network analysis suggested that some differentially expressed genes were involved in spermatogenesis through ECM-receptor interaction, and PI3K-Akt signaling pathways. Our data provide novel insights into the molecular expression and regulation similarities and diversities in spermatogenesis and testicular development in yaks at different stages of sexual maturity.
Subject
Cell Biology,Obstetrics and Gynecology,Endocrinology,Embryology,Reproductive Medicine
Cited by
18 articles.
订阅此论文施引文献
订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献