Relationships of changes in B-mode echotexture and colour-Doppler signals in the wall of the preovulatory follicle to changes in systemic oestradiol concentrations and the effects of human chorionic gonadotrophin in mares

Abstract

A duplex grey-scale and colour-Doppler ultrasound instrument was used to study the changes in the wall of the preovulatory follicle in mares. When the follicle reached ≥35 mm (hour 0), mares were randomized into control (n= 16) and human chorionic gonadotropin (hCG)-treated (n= 16) groups. The hCG treatment was given at hour 0. Scanning was done every 12 h until hour 36, every hour between hours 36 and 48, and every 12 h thereafter until ovulation. Blood was sampled every 12 h for oestradiol assay. During the period 0–24 h post-treatment, oestradiol concentrations decreased in the hCG group and increased in the controls (significant interaction). During the period 0–36 h post-treatment, thickness and echogenicity of the granulosa increased in the hCG group but not in the controls. During the period 36 to 12 h before ovulation, granulosa and colour-Doppler end-points increased in the control and hCG groups (hour effects), while oestradiol was decreasing in both groups. The prominence and percentage of follicle circumference with an anechoic band peripheral to the granulosa and colour-Doppler signals in the follicle wall, indicating arterial blood flow, decreased during the period 4 to 1 h before ovulation (hour effects). Results indicated that the ultrasonographic changes of the wall of the preovulatory follicle were not associated temporally with changes in oestradiol concentrations and prominence of an anechoic band, and colour-Doppler signals decreased during the few hours before ovulation. The hypothesis that the latter portion of the ovulatory LH surge has a negative effect on systemic oestradiol was supported by the immediate decrease in oestradiol concentrations when hCG was injected.