Lateral force separation of biopolymers using an atomic force microscope

Author:

Anderson Mark S.1ORCID

Affiliation:

1. Jet Propulsion Laboratory, California Institute of Technology , 4800 Oak Grove Drive, M/S 125-109, Pasadena, California 91109, USA

Abstract

The lateral force separation of long chain biomolecules is demonstrated using an atomic force microscope (AFM). This is achieved by using an AFM tip to pull molecules away from the edge of a nanofluidic solution. By monitoring the torsion on the AFM cantilever, a characteristic force–distance signal is produced when long chain molecules separate and detach from the solvent edge. This lateral force separation using AFM (LFS-AFM) is demonstrated on egg albumin proteins and synthetic DNA strands. The detected length of the protein and nucleotide biopolymers was consistent with their calculated molecular contour length. LFS AFM provides separation and detection of single polymer strands that has potential applications in biochemical analysis, paleontology, and life detection.

Publisher

AIP Publishing

Subject

Condensed Matter Physics,General Materials Science,Fluid Flow and Transfer Processes,Colloid and Surface Chemistry,Biomedical Engineering

Reference31 articles.

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