1. We used dilute Michaelis buffers of the following composition: the basic buffer contained 4.85 g of sodium acetate and 7.35 g of barbital sodium per liter of solution. The acid buffer was obtained from the basic buffer by adding to 100 cc of it 75 cc of N∕10 HCl and 285 cc of water.
2. It is noteworthy that with some proteins local laminar concentrations or precipitations are observed before the current is passed. It is to be expected that concentration variations should occur in a nonuniformpH field for substances whose solubility and intermolecular forces depend onpH. (Note the slight darkening near the center of the left arm in Fig. 2D.)