Nanogap traps for passive bacteria concentration and single-point confocal Raman spectroscopy

Author:

Han Jung Y.1ORCID,Yeh Michael1ORCID,DeVoe Don L.1ORCID

Affiliation:

1. Department of Mechanical Engineering, University of Maryland , College Park, Maryland 20742, USA

Abstract

A microfluidic device enabling the isolation and concentration of bacteria for analysis by confocal Raman spectroscopy is presented. The glass-on-silicon device employs a tapered chamber surrounded by a 500 nm gap that serves to concentrate cells at the chamber apex during sample perfusion. The sub-micrometer gap retains bacteria by size exclusion while allowing smaller contaminants to pass unimpeded. Concentrating bacteria within the fixed volume enables the use of single-point confocal Raman detection for the rapid acquisition of spectral signatures for bacteria identification. The technology is evaluated for the analysis of E. cloacae, K. pneumoniae, and C. diphtheriae, with automated peak extraction yielding distinct spectral fingerprints for each pathogen at a concentration of 103 CFU/ml that compare favorably with spectra obtained from significantly higher concentration reference samples evaluated by conventional confocal Raman analysis. The nanogap technology offers a simple, robust, and passive approach to concentrating bacteria from dilute samples into well-defined optical detection volumes, enabling rapid and sensitive confocal Raman detection for label-free identification of focused cells.

Funder

National Institute of Allergy and Infectious Diseases

Publisher

AIP Publishing

Subject

Condensed Matter Physics,General Materials Science,Fluid Flow and Transfer Processes,Colloid and Surface Chemistry,Biomedical Engineering

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