Investigating the reliability of the evoked response in human iPSCs-derived neuronal networks coupled to micro-electrode arrays

Author:

Zanini Giorgia1ORCID,Parodi Giulia1ORCID,Chiappalone Michela12ORCID,Martinoia Sergio1ORCID

Affiliation:

1. Department of Informatics, Bioengineering, Robotics, and Systems Engineering (DIBRIS), University of Genova 1 , Genova, Italy

2. IRCCS Ospedale Policlinico San Martino 2 , Genova, Italy

Abstract

In vitro models of neuronal networks have emerged as a potent instrument for gaining deeper insights into the intricate mechanisms governing the human brain. Notably, the integration of human-induced pluripotent stem cells (hiPSCs) with micro-electrode arrays offers a means to replicate and dissect both the structural and functional elements of the human brain within a controlled in vitro environment. Given that neuronal communication relies on the emission of electrical (and chemical) stimuli, the employment of electrical stimulation stands as a mean to comprehensively interrogate neuronal assemblies, to better understand their inherent electrophysiological dynamics. However, the establishment of standardized stimulation protocols for cultures derived from hiPSCs is still lacking, thereby hindering the precise delineation of efficacious parameters to elicit responses. To fill this gap, the primary objective of this study resides in delineating effective parameters for the electrical stimulation of hiPSCs-derived neuronal networks, encompassing the determination of voltage amplitude and stimulation frequency able to evoke reliable and stable responses. This study represents a stepping-stone in the exploration of efficacious stimulation parameters, thus broadening the electrophysiological activity profiling of neural networks sourced from human-induced pluripotent stem cells.

Funder

Ministero dell'Istruzione, dell'Università e della Ricerca

Publisher

AIP Publishing

Subject

Biomedical Engineering,Biomaterials,Biophysics,Bioengineering

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