Construction of an acellular spinal cord scaffold with a novel NT-3 sustained-release system promotes the neural differentiation of BMSCs through the NT-3/TrkC pathway

Author:

Jiang Tao1,Yin Hong1,Yu Miao1,Wang Han2,Xing Hui3ORCID

Affiliation:

1. Department of Orthopedics, Xinqiao Hospital, Army Medical University 1 , Chongqing 400037, China

2. Department of Orthopedics, The First Affiliated Hospital of Chongqing Medical University 2 , Chongqing 400016, China

3. Department of Center for Orthopedics and Trauma, The Third Affiliated Hospital of Chongqing Medical University 3 , Chongqing 401120, China

Abstract

In previous neural tissue engineering studies, we successfully constructed NT-3 cross-linked acellular spinal cord scaffolds (NT-3 cross-linked scaffolds), which can sustain the release of NT-3 and promote the differentiation of rat bone marrow mesenchymal stem cells (BMSCs) into neuron-like cells. However, the molecular mechanism by which NT-3 cross-linked scaffolds promote BMSC differentiation into neurons is unknown, coupled with the low drug loading of scaffolds and the sudden release of NT-3 on the first day. We used WB and PCR in combination with NT-3/TrkC, MAPK/ERK, and PI3K/Akt pathway inhibitors to determine the mechanism of action in vitro. We hypothesized that NT-3 mediates the NT-3/TrkC pathway as a major target molecule that promotes the differentiation of BMSCs into neurons. We prepared an improved NT-3 scaffold and improve the sustained release of NT-3 through the combination of heparin methacryloyl and EDC/NHS. The adhesion, proliferation, differentiation, and NT-3/TrkC signaling pathway of BMSCs on different scaffolds were analyzed. We concluded that NT-3-improved scaffolds can be loaded with more NT-3 and more effectively promote the differentiation of BMSCs into neurons through the NT-3/TrkC pathway. The proposed method has biocompatibility and provides a new idea for spinal cord repair.

Funder

Natural Science Foundation Project of Chongqing, Chongqing Science and Technology Commission

Science and Technology Planning Project of Yubei District, Chongqing

Publisher

AIP Publishing

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