Carbon nanotube substrates enhance SARS-CoV-2 spike protein ion yields in matrix-assisted laser desorption–ionization mass spectrometry-Reference-Cited by-同舟云学术

Carbon nanotube substrates enhance SARS-CoV-2 spike protein ion yields in matrix-assisted laser desorption–ionization mass spectrometry

Published:2023-01-30 Issue:5 Volume:122 Page:
ISSN:0003-6951
Container-title:Applied Physics Letters
language:en
Short-container-title:

Author:

Schenkel T.¹^ORCID,Snijders A. M.²^ORCID,Nakamura K.¹^ORCID,Seidl P. A.¹,Mak B.¹,Obst-Huebl L.¹^ORCID,Knobel H.³^ORCID,Pong I.¹^ORCID,Persaud A.¹^ORCID,van Tilborg J.¹^ORCID,Ostermayr T.¹,Steinke S.¹^ORCID,Blakely E. A.¹^ORCID,Ji Q.¹^ORCID,Javey A.⁴^ORCID,Kapadia R.⁵^ORCID,Geddes C. G. R.¹^ORCID,Esarey E.¹

Affiliation:

1. Accelerator Technology and Applied Physics Division, Lawrence Berkeley National Laboratory 1 , Berkeley, California 94720, USA

2. Biological Systems and Engineering Division, Lawrence Berkeley National Laboratory 2 , Berkeley, California 94720, USA

3. Eurofins Materials Science Netherlands BV 3 , 5656 AE Eindhoven, The Netherlands

4. Electrical Engineering and Computer Science Department, University of California 4 , Berkeley, California 94720, USA

5. School of Engineering, University of Southern California 5 , Los Angeles, California 90089, USA

Abstract

Nanostructured surfaces enhance ion yields in matrix-assisted laser desorption–ionization mass spectrometry (MALDI-MS). The spike protein complex, S1, is one fingerprint signature of Sars-CoV-2 with a mass of 75 kDa. Here, we show that MALDI-MS yields of Sars-CoV-2 spike protein ions in the 100 kDa range are enhanced 50-fold when the matrix–analyte solution is placed on substrates that are coated with a dense forest of multi-walled carbon nanotubes, compared to yields from uncoated substrates. Nanostructured substrates can support the development of mass spectrometry techniques for sensitive pathogen detection and environmental monitoring.

Funder

DOE FES

DOE HEP

LBNL LDRD

Publisher

AIP Publishing

Subject

Physics and Astronomy (miscellaneous)

Link

https://pubs.aip.org/aip/apl/article-pdf/doi/10.1063/5.0128667/16732980/050601_1_online.pdf

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