Xanthophyll-cycle based model of the rapid photoprotection of <i>Nannochloropsis</i> in response to regular and irregular light/dark sequences-Reference-Cited by-同舟云学术

Xanthophyll-cycle based model of the rapid photoprotection of Nannochloropsis in response to regular and irregular light/dark sequences

Published:2022-05-28 Issue:20 Volume:156 Page:205102
ISSN:0021-9606
Container-title:The Journal of Chemical Physics
language:en
Short-container-title:J. Chem. Phys.

Author:

Short Audrey H.¹²³^ORCID,Fay Thomas P.⁴^ORCID,Crisanto Thien²⁵,Hall Johanna⁶,Steen Collin J.²³⁴^ORCID,Niyogi Krishna K.²⁵⁷^ORCID,Limmer David T.¹³⁴⁸⁹^ORCID,Fleming Graham R.¹²³⁴^ORCID

Affiliation:

1. Graduate Group in Biophysics, University of California, Berkeley, California 94720, USA

2. Molecular Biophysics and Integrated Bioimaging, Division Lawrence Berkeley National Laboratory, Berkeley, California 94720, USA

3. Kavli Energy Nanoscience Institute, Berkeley, California 94720, USA

4. Department of Chemistry, University of California, Berkeley, California 94720, USA

5. Department of Plant and Microbial Biology, University of California, Berkeley, California 94720, USA

6. Department of Environmental Engineering, Georgia Institute of Technology, Atlanta, Georgia 30332, USA

7. Howard Hughes Medical Institute, University of California, Berkeley, California 94720, USA

8. Chemical Science Division, Lawrence Berkeley National Laboratory, Berkeley, California 94720, USA

9. Material Science Division, Lawrence Berkeley National Laboratory, Berkeley, California 94720, USA

Abstract

We explore the photoprotection dynamics of Nannochloropsis oceanica using time-correlated single photon counting under regular and irregular actinic light sequences. The varying light sequences mimic natural conditions, allowing us to probe the real-time response of non-photochemical quenching (NPQ) pathways. Durations of fluctuating light exposure during a fixed total experimental time and prior light exposure of the algae are both found to have a profound effect on NPQ. These observations are rationalized with a quantitative model based on the xanthophyll cycle and the protonation of LHCX1. The model is able to accurately describe the dynamics of non-photochemical quenching across a variety of light sequences. The combined model and observations suggest that the accumulation of a quenching complex, likely zeaxanthin bound to a protonated LHCX1, is responsible for the gradual rise in NPQ. Additionally, the model makes specific predictions for the light sequence dependence of xanthophyll concentrations that are in reasonable agreement with independent chromatography measurements taken during a specific light/dark sequence.

Funder

U.S. Department of Energy, Office of Science, Basic Energy Sciences, Chemical Sciences, Geosciences, and Biosciences Division

U.S. Department of Energy, Office of Science, Basic Energy Sciences, CPIMS Program Early Career Research Program

Howard Hughes Medical Institute

DOE Science Undergraduate Laboratory Internship, Lawrence Berkeley National Laboratory

Publisher

AIP Publishing

Subject

Physical and Theoretical Chemistry,General Physics and Astronomy

Link

https://aip.scitation.org/doi/pdf/10.1063/5.0089335

Reference36 articles.

1. Enzymes and Mechanisms for Violaxanthin-zeaxanthin Conversion

2. Photoprotection and Other Responses of Plants to High Light Stress

3. Sensing and Responding to Excess Light

4. Crops on the fast track for light

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