Opto-electromechanical quantification of epithelial barrier function in injured and healthy airway tissues

Author:

Chen Jiawen1ORCID,Mir Seyed Mohammad1ORCID,Hudock Maria R.2ORCID,Pinezich Meghan R.2,Chen Panpan23ORCID,Bacchetta Matthew45ORCID,Vunjak-Novakovic Gordana2ORCID,Kim Jinho1ORCID

Affiliation:

1. Department of Biomedical Engineering, Stevens Institute of Technology 1 , Hoboken, New Jersey 07030, USA

2. Department of Biomedical Engineering, Columbia University 2 , New York, New York 10027, USA

3. Department of Surgery, Columbia University 3 , New York, New York 10027, USA

4. Department of Cardiac Surgery, Vanderbilt University 4 , Nashville, Tennessee 37235, USA

5. Department of Biomedical Engineering, Vanderbilt University 5 , Nashville, Tennessee 37235, USA

Abstract

The airway epithelium lining the luminal surface of the respiratory tract creates a protective barrier that ensures maintenance of tissue homeostasis and prevention of respiratory diseases. The airway epithelium, unfortunately, is frequently injured by inhaled toxic materials, trauma, or medical procedures. Substantial or repeated airway epithelial injury can lead to dysregulated intrinsic repair pathways and aberrant tissue remodeling that can lead to dysfunctional airway epithelium. While disruption in the epithelial integrity is directly linked to degraded epithelial barrier function, the correlation between the structure and function of the airway epithelium remains elusive. In this study, we quantified the impact of acutely induced airway epithelium injury on disruption of the epithelial barrier functions. By monitoring alternation of the flow motions and tissue bioimpedance at local injury site, degradation of the epithelial functions, including mucociliary clearance and tight/adherens junction formation, were accurately determined with a high spatiotemporal resolution. Computational models that can simulate and predict the disruption of the mucociliary flow and airway tissue bioimpedance have been generated to assist interpretation of the experimental results. Collectively, findings of this study advance our knowledge of the structure–function relationships of the airway epithelium that can promote development of efficient and accurate diagnosis of airway tissue injury.

Funder

National Science Foundation

New Jersey Health Foundation

American Thoracic Society

National Institutes of Health

Publisher

AIP Publishing

Subject

Biomedical Engineering,Biomaterials,Biophysics,Bioengineering

同舟云学术

1.学者识别学者识别

2.学术分析学术分析

3.人才评估人才评估

"同舟云学术"是以全球学者为主线,采集、加工和组织学术论文而形成的新型学术文献查询和分析系统,可以对全球学者进行文献检索和人才价值评估。用户可以通过关注某些学科领域的顶尖人物而持续追踪该领域的学科进展和研究前沿。经过近期的数据扩容,当前同舟云学术共收录了国内外主流学术期刊6万余种,收集的期刊论文及会议论文总量共计约1.5亿篇,并以每天添加12000余篇中外论文的速度递增。我们也可以为用户提供个性化、定制化的学者数据。欢迎来电咨询!咨询电话:010-8811{复制后删除}0370

www.globalauthorid.com

TOP

Copyright © 2019-2024 北京同舟云网络信息技术有限公司
京公网安备11010802033243号  京ICP备18003416号-3