Affiliation:
1. Center for Advanced Reaction Dynamics, Institute for Basic Science, Daejeon 34141, South Korea
2. Department of Chemistry and KI for the BioCentury, Korea Advanced Institute of Science and Technology (KAIST), Daejeon 34141, South Korea
Abstract
The structure of a protein is closely related to its biological function. In this regard, structural changes, as well as static structures, have been scrutinized as essential elements in understanding and controlling the function of a protein. In particular, the structural change in the solution phase needs to be elucidated to properly understand protein functions under physiological conditions. Time-resolved x-ray liquidography (TRXL), also known as time-resolved x-ray solution scattering, has attracted attention as a powerful experimental method for studying the structural dynamics of proteins in the solution phase. Initially, TRXL was used to study the structural dynamics of small molecules in the solution phase, and later, its application was extended to probe the structural changes in proteins. Via TRXL, structural changes ranging from large quaternary movements to subtle rearrangements of the tertiary structures have been successfully elucidated. In this review, we introduce various studies using TRXL to investigate the structural dynamics of proteins. These include early TRXL studies on model systems, those on photoreceptor proteins, and recent studies using stimuli beyond the direct photoexcitation of proteins.
Funder
Institute for Basic Science
Subject
General Earth and Planetary Sciences,General Engineering,General Environmental Science
Cited by
3 articles.
订阅此论文施引文献
订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献