Bactericidal effectiveness of high-intensity pulsed broadband irradiation in treating infected wounds

Abstract

The study aimed to investigate the bactericidal eŠcacy of high-intensity pulsed broadband irradiation in the treatment of infected wounds. An experimental study was conducted on 90 mature male Wistar rats. An infected wound model was created by contaminating with Staphylococcus aureus, Pseudomonas aeruginosa, Klebsiella pneumoniae, and Candida albicans. Animals in Group 1 received high-intensity pulsed broadband irradiation. Animals in Group 2 received traditional UV irradiation. Animals in Group 3 had their wounds cleaned with 0.1% chlorhexidine solution. By the 3rd day of treatment, animals that received pulsed high-intensity broadband irradiation showed a signifiant reduction in contamination by Staphylococcus aureus, Klebsiella pneumoniae, and Pseudomonas aeruginosa compared to Group 3. By the 7th day of treatment, half or the majority of animals in Groups 1 and 2 showed complete decontamination of wounds concerning Staphylococcus aureus and Klebsiella pneumoniae. Most animals in Group 1 showed complete wound clearance of Pseudomonas aeruginosa. By the 10th day, nearly all animals in Group 1 demonstrated complete decontamination of wounds. Statistical analysis revealed a signifiant difference in the reduction of wound contamination with Staphylococcus aureus and Klebsiella pneumoniae by the 10th day in Groups 1 and 2 compared to Group 3. Thus, the use of high-intensity pulsed broadband irradiation of wounds reduces the degree of pathogenic microorganism contamination in a shorter time frame.