Optical spectroanalyzer with extended dynamic range for pharmacokinetic investigations of photosensitizers in biotissue
-
Published:2019-03-31
Issue:1
Volume:8
Page:46-51
-
ISSN:2413-9432
-
Container-title:Biomedical Photonics
-
language:
-
Short-container-title:Biomedical photonics
Author:
Meerovich G. A.1, Akhlyustina E. V.2, Savelieva T. A.1, Linkov K. G.3, Loschenov V. B.1
Affiliation:
1. Prokhorov General Physics Institute of the Russian Academy of Sciences, Moscow; National Research Nuclear University MEPhI (Moscow Engineering Physics Institute), Moscow 2. National Research Nuclear University MEPhI (Moscow Engineering Physics Institute), Moscow 3. Prokhorov General Physics Institute of the Russian Academy of Sciences, Moscow
Abstract
Currently, the most promising method for the study of pharmacokinetics of drugs with fluorescent properties is the spectral-fluorescent method. In this article, we propose an algorithm for expanding the dynamic range of the spectrum analyzer by automatically monitoring the maximum spectral density in the recorded fluorescence spectrum and automatically controlled changes in the accumulation time depending on this value, followed by compensation of the output signal with regard to this change, as well as hardware circuit solutions that allow this algorithm.Testing of LESA-01-"Biospeс" spectrum analyzer, upgraded using the proposed approach, was carried out on photosensitizer dispersions based on tetra-3-phenylthiophthalocyanine hydroxyaluminium of various concentrations (from 0.01 mg/l to 50 mg/l), approximately corresponding to the concentrations realized in the process of studying pharmacokinetics in calibration samples and tissues of experimental animals.The proposed solutions that implement the algorithm for recording fluorescence spectra with automatic change of accumulation time depending on the signal level, ensured a significant expansion of the dynamic range of the spectrum analyzer (up to 3.5 orders of magnitude) and improved accuracy in pharmacokinetic studies.
Publisher
Russian Photodynamic Association
Subject
Dermatology,Surgery
Reference5 articles.
1. Rukovodstvo po provedeniyu doklinicheskih issledovaniy lekarstvennyh sredstv. CHast' pervaya [A guide to preclinical drug research. Part One], ed. A.N. Mironov. Moscow, Grif and K Publ., 2012. 944 p. 2. Loschenov V.B., Linkov K.G., Savelieva T.A., Loschenov M.V., Model S.S., Borodkin A.V. Hardware and tool equipment for fluorescence diagnostics and photodynamic therapy, Photodynamic therapy and photodyagnosis, 2013, vol. 2, no. 3, pp. 17–25. (In Russian) 3. Meerovich G.А., Tiganova I.G., Makarova E.А., Meerovich I.G., Romanova Ju.М., Tolordava E.R. , Alekseeva N.V., Stepanova Т.V., Koloskova Yu.S., Lukyanets Е.А., Krivospitskaya N.V., Sipailo I.P., Baikova Т.V., Loschenov V.B., Gonchukov S.A. Photodynamic inactivation of bacteria and biofilms using cationic bacteriochlorins, J. Phys. Conf. Ser., 2016, vol. 691, 012011. 4. Meerovich G.A., Akhlyustina E.V., Tiganova I.G., Panov V.A., Tyukova V.S., Тolordava E.R., Alekseeva N.V., Linkov K.G., Romanova Yu.M., Grin M.A., Mironov A.F.4, Loschenov V.B., Kaprin A.D., Filonenko E.V. Study of photosensitizer for antibacterial photodynamic therapy based on cyclodextrin formulation of 133-N-(Nmethylnicotinyl)- bacteriopurpurinimide methyl ester, Biomedical Photonics, 2017, vol. 6, no. 3, pp. 16–32. (in Russian) 5. Akhlyustina E.V., Bud’ko А.P., Lantsova A.V., Lin’kov K.G., Loschenov V.B., Savelieva T.A. Ustroystvo dlya spektral'no-fluorestsentnogo issledovaniya soderzhaniya fluorohromov [Device for spectral fluorescence study of the content of fluorochromes], Patent RF no. 2665628, 2018.
Cited by
4 articles.
订阅此论文施引文献
订阅此论文施引文献，注册后可以免费订阅5篇论文的施引文献，订阅后可以查看论文全部施引文献
|
|