Affiliation:
1. Airlangga University
2. Sumatera Institute of Technology
3. Sepuluh Nopember Institute of Technology
4. Universiti Teknologi Malaysia
Abstract
Photodynamic inactivation is an effective treatment that uses light irradiation, photosensitizer and oxygen. The aim of this study was to determine photodynamic effectiveness of laser diode combined with ozone to reduce Staphylococcus aureus biofilm using exogenous chlorophyll (Chlo). The chlorophyll was extracted from leave of Dracaena angustifolia. To determine the antibacterial effect of S. aureus biofilm treatments, samples were separated into Chlo, Laser, Chlo+Laser, Ozone, Ozone+Laser, Chlo+Ozone+Laser categories. The data were analyzed using ANOVA test. The result of this study showed that Chlo+Ozone+Laser combine treatment at 20 s exposure of ozone with 4 min of irradiation time lead to 80.26 % reduction of biofilm activity, which was the highest efficacy of all the treatment groups. The combination of laser, chlorophyll and lower ozone concentration increases the effectiveness of photodynamic inactivation.
Publisher
Russian Photodynamic Association
Reference29 articles.
1. Donlan R.M. Biofilms and Device-Associated Infections, Emerg. Infect. Dis., 2001, vol. 7(2), pp. 277–281.
2. Gordon R.J., Lowy F.D. Pathogenesis of Methicillin-Resistant Staphylococcus aureus Infection, Clin. Infect. Dis., 2008, vol. 46(5), pp. 350–359.
3. Stewart P.S., Costerton J.W. Antibiotic resistance of bacteria in biofilms, Lancet, 2001, vol. 358(9276), pp. 135–138.
4. Oli A.K., Raju S., Nagaveni S. Biofilm formation by Multidrug resistant Enterococcus faecalis (MDEF) originated from clinical samples Ella foundations Hyderabad, J. Microbiol. Biotechnol. Res., 2012, vol. 2(2), pp. 284–288.
5. Xu Y., Itzek A., Kreth J. Comparison of genes required for H2O2 resistance in Streptococcus gordonii and Streptococcus sanguinis, Microbiology, 2014, vol. 160, pp. 2627–2638.
Cited by
19 articles.
订阅此论文施引文献
订阅此论文施引文献，注册后可以免费订阅5篇论文的施引文献，订阅后可以查看论文全部施引文献