Genetic analyzer Nanophore 05 as a measuring instrument for DNA sequencing-Reference-Cited by-同舟云学术

Genetic analyzer Nanophore 05 as a measuring instrument for DNA sequencing

Published:2021 Issue:1 Volume: Page:60-65
ISSN:0368-1025
Container-title:Izmeritel`naya Tekhnika
language:
Short-container-title:Izmer. Tekhn.

Author:

Volkov Aleksandr A.¹,Volkov Il’ya A.²,Plugov Aleksandr G.²,Kulyabina Elena V.³,Melkova Olga N.³,Lavrov Georgii S.⁴,Bocharova Dariya V.⁴,Alekseev Yakov I.⁵

Affiliation:

1. Russian Federal Research Institute of Fisheries and Oceanography

2. Limited Liability Company Syntol

3. All-Russian Research Institute of Metrological Service

4. Institute for Analytical Instrumentation

5. Limited Liability Company Syntol; Institute for Analytical Instrumentation

Abstract

The paper presents the results of studies on the development of metrological support for DNA sequencing. The sequencing of a standard sample of the human mitochondrial DNA was carried out according to the Sanger method on domestic capillary electrophoresis genetic analyzers Nanophore 05. The results of the work were used to validate standard reference material for human mitochondrial DNA sequence and to confirm the DNA sequencer as a measurement instrument.

Publisher

FSUE VNIIMS All-Russian Research Institute of Metrological Service

Subject

General Medicine

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