Molecular Detection of Some Anaplasma Species in Blood of Dogs in Baghdad Province, Iraq

Abstract

A total of 150 blood samples were collected from dogs and examined by the polymerase chain reaction (PCR) technique, which was used to detect the 16S RNA gene of Anaplasma platys and Anaplasma phagocytophilum. Subsequent analysis of the PCR amplicons was achieved by nucleotides sequencing of some positive samples. Totally, the findings show the presence of PCR products (i.e., Anaplasma spp. infection) in 12/150 (8.0%) of the dogs under study. While 5/150 (3.33%) of the cases were A. platys, 7/150 (4.66%) were A. phagocytophilum. Nucleotide sequencing confirmed the identity of the amplified genes whose sequences were compared with other references belong to 15 of 16S rRNA gene of A. platys and 14 references of 16S rRNA gene of A. phagocytophilum, and the isolate sequences of this study were deposited on the Gene Bank. The identity and similarity scores between the isolates of this study and reference strains ranged from 98 to 99%. In conclusion, canine anaplasmosis prevalence in dogs could be underestimated in Iraq, and the phylogenetic tree of the local A. platys and A. phagocytophilum isolates were found to resemble other worldwide strains of Anaplasma spp. with a high degree of similarity.