Abstract
The mechanisms of anticancer action of laser radiation in the red range of the spectrum (wavelength 650 nm) with a power
of 50 mW are studied. Irradiation of rats was carried out with a laser diode through the skin into the area of growth of
Guerin's carcinoma. Guerin's carcinoma was transplanted by injecting 0.5 ml of a 30% suspension of cancer cells in saline
into the thigh of the right limb. Animals were divided into four groups: group I – intact animals (control); group II – rats,
which were irradiated daily for 4 minutes in the thigh of the right limb; group III - rats with transplanted Guerin's carcinoma;
group IV - tumor-bearing rats, which were exposed to laser radiation in the area of tumor growth. Euthanasia of animals was
performed under light ether anesthesia on the 14th and 21st day of tumor growth in the body.For the study of the blood
coagulation system of rats used biochemical coagulogram with the analysis of the following indicators - the content of
fibrinogen, thrombin and prothrombin time, activated partial thromboplastin time (APTT), platelet count. Analysis of blood
smears was performed using a light microscope. It was found that the daily four-minute action of laser irradiation in the thigh
of the right limb does not lead to changes in the hemacoagulation system. The growth of Guerin's carcinoma in the body leads
to a decrease in the concentration of factor I blood clotting (fibrinogen) and an increase in thrombin time. At the same time, a
decrease in prothrombin time and APTT was detected, which indicates hypercoagulation processes that occur due to II, V,
VII, VIII, IX, X, XI, XII coagulation factors, which can negatively affect the general condition of the body. The changes we
detected in the blood smears were manifested by erythrocyte adhesion, platelet depletion, lymphocyte disintegration,
hypersegmentation of segmental neutrophils, and the appearance of Gumprecht shadows, which are remnants of destroyed
lymphocytes. The four-minute action of the laser diode on the growth site of Guerin's carcinoma improves the clinical and
biochemical parameters of the rat blood coagulation system in the logarithmic and stationary phases of oncogenesis.
Publisher
Yuriy Fedkovych Chernivtsi National University