STATE OF THE XENOBIOTICS BIOTRANSFORMATION SYSTEM IN THE LIVER MICROSOMAL FRACTION OF RATS UNDER THE EFFECTS OF SODIUM BENZOATE AND ASCORBIC ACID

Abstract

The combined effect of food preservatives – sodium benzoate and ascorbic acid on the state of monooxygenase system (MOS) components in the microsomal fraction of rat liver cells are studied in the paper. Changes in the level of cytochrome P450 and cytochrome b5, as well as the rate of reduction-oxidation of these hemoproteins in the microsomal fraction of the rat liver under conditions of administration of sodium benzoate and ascorbic acid were studied. During the experiment, rats were divided into four groups: group I – control (intact animals); group II – rats that were injected with ascorbic acid at a dose of 30 mg per kg of animal weight; group III – rats that were injected with sodium benzoate at a dose of 750 mg per kg of animal weight; group IV – rats that were injected with sodium benzoate 30 minutes before the introduction of ascorbic acid. Sodium benzoate and ascorbic acid were administered per os daily for 21 days. Animals were euthanized under light ether anesthesia on the 21st day after the start of administration of sodium benzoate and ascorbic acid. It was established that the three-week introduction of sodium benzoate into the body of animals leads to a decrease in the level of cytochrome P450, which occurs due to an increase in the rate of transition of cytochrome P450 into its inactive form P420. It was shown that along with the decrease in cytochrome P450 in the microsomal fraction of the liver, the level of cytochrome b5 decreases with a simultaneous increase in the rate of reduction and oxidation of this hemoprotein. Sodium benzoate exhibits a higher destructive effect when it is combined with ascorbic acid, which is expressed by a decrease in the level of MOS hemoproteins and may be a consequence of dangerous metabolites formation in the body - benzoic acid and benzene.