The changes of activity of separate carbohydrate metabolism enzymes in blood erythrocytes of pregnant rats under vanadium citrate

Abstract

The aim of the work was to study the effect of vanadium citrate on the activity of glucose-6-phosphate dehydrogenase, lactate dehydrogenase, estradiol content and to establish correlations between the activity of the studied enzymes and the estradiol content in pregnant female rats under the effect of vanadium compound. The study was carried out on 25 female Wistar rats, divided into five groups: group I - non-pregnant, group II - pregnant consuming pure water without additives, groups III, IV, V - females which during the mating and pregnancy periods received the solution of vanadium citrate at the concentrations of 3.75, 15.63 and 62.5 μgV/kg of body weight, respectively. Glucose-6-phosphate dehydrogenase, lactate dehydrogenase and estradiol content increase in pregnant females of group II compared to group I of non-pregnant animals. In pregnant females, the effect of vanadium citrate resulted in the decrease in the activity of glucose-6-phosphate dehydrogenase and lactate dehydrogenase in the blood of animals in groups IV and V, and the content of estradiol in the animals of all three experimental groups (III, IV, V), compared to group II of pregnant females. The values of glucose-6-phosphate dehydrogenase activity in the animals of group IV, which received the solution of vanadium citrate at the concentration of 15.63 μgV/kg of body weight, was similar to that of the animals in group I. This may indicate the normalizing effect of vanadium citrate on the activity of glucose-6-phosphate dehydrogenase at the indicated concentration, which is probably due to the ability of the vanadium microelement to inhibit the activity of this enzyme. Vanadium citrate at the concentration of 15.63 μgV/kg of body weight caused the recovery of the enzyme activity to the values of the activity in group І.