The use of lactic acid bacteria as probiotics as a composition of live feed

Abstract

The losses in the conditions of intensive aquaculture can be linked with the development of a range of bacterial and viral infectious diseases of hydrobionts. Probiotics are used as the means of treatment and prevention of the dysbiosis of fish organisms. The search for new probiotic cultures and their subsequent trials, the possibility of their co-culturing, using this scientific foundation for devising combined preventative and therapeutic means and their further bioencapsulation in freshwater zooplankton will enable researchers to increase the resistance and responsiveness of fish organisms at the different stages of ontogenesis. The lactic acid bacteria are characterized as being toxic-free and having a wide range of antagonistic activity, namely the development of the colonization resistance of mucous membranes and coats owing to the generation of short-chain fatty acids, bacteriocins, lytic enzymes, antibiotic combinations, the decrease of pH index. This research is dedicated to the elucidation of new probiotic micro-organisms and the possibility of their integration into zooplankton aiming at their further use as the nutritious substrate for fish in the conditions of aquaculture. The thesis presents the results of the conducted analysis of the adhesive properties of probiotic cultures, determines their sensitivity to natural inhibitors as well as the main groups of antibiotics and evaluates the effectiveness of bioencapsulation of bacteria in the organisms of small-sized freshwater crustaceans. The bioencapsulation of mono- or combined probiotics in the organisms of daphnia has increased their survival rates, the length of the bodies of individual representatives of the species and hasn’t had a significant impact upon the correlation of the basic nutrients, which has been established in the course of the description of the biomass chemical content of small-sized crustaceans.