Affiliation:
1. From the Department of Biochemistry, University of Toronto, Canada
Abstract
1. An experimental system suitable for the study of enzyme formation has been described.
2. The formation of ß-galastosidase in E. coli B could be induced by lactose, melibiose, D-galactose and ß-methyl-D-galactoside.
3. Lactose-induced ß-galactosidase formation was found to be inhibited by D-glucose, D-mannose, D-fructose, D-arabinose, and raffinose.
4. The utilizable structural analogue, D-glucose, was found to either stimulate or inhibit ß-galactosidase formation depending upon its concentration. D-Arabinose, on the other hand, a non-utilizable structural analogue, is only capable of inhibiting, whereas succinic acid, a structurally unrelated energy source, is only capable of stimulating ß-galactosidase formation.
5. D-Arabinose inhibition of lactose-induced ß-galactosidase formation was found to be of the competitive type.
6. Some of the implications of these findings have been discussed.
Publisher
Rockefeller University Press
Cited by
37 articles.
订阅此论文施引文献
订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献
1. Biochemistry of α-Galactosidases;Advances in Enzymology - and Related Areas of Molecular Biology;2006-11-22
2. The Induced Synthesis of Proteins;Advances in Enzymology - and Related Areas of Molecular Biology;2006-11-22
3. Induction of β-Galactosidase in
Lactobacillus plantarum;Journal of Bacteriology;1974-10
4. Induction and Characterization of β-Galactosidase in an Extreme Thermophile;Journal of Bacteriology;1972-05
5. The α-galactosidase from Escherichia coli K12;Biochimica et Biophysica Acta (BBA) - General Subjects;1971-01